Aptamers are best known as protein ligands, rivaling antibody performance in both affinity and specificity. However, since the technology was first introduced in 1990, thousands of academic papers have published results showing aptamers can bind nearly every type of molecule; including small organic molecules, cellular toxins, viruses and targets as small as heavy metal ions.
In most applications, aptamers have successfully replaced unreliable or unsuitable antibody reagents. Their use in aptamer westerns and dot blots is well supported in academic literature. Numerous publications support the feasibility of aptamer integration into biosensors, such as lead sensors, drug sensors and estrogen measuring devices. They can be used in single-target quantitative assays or multi-target arrays.
For applications such as protein detection, Base Pair Biotechnologies can custom conjugate Bovine Serum Albumin (BSA), Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) to aptamers upon request. Reporter and functional groups can be attached in a deterministic way. ADME features can be fine-tuned through conjugating aptamers to other groups, such as polyethylene glycol (PEG). Aptamers can even be combined with antibodies for complex drug delivery. Recently, aptamers have been used as chimeric conjugates to siRNAs.
Aptamers are a promising form of synthetic, inexpensive affinity reagent that can be employed in numerous applications. Despite their promise, however, they have generally only been developed or ‘evolved’ against a single target at a time. High costs for aptamer selection (as well as past intellectual property constraints) have hindered their widespread use.
Base Pair Biotechnologies’ novel approach to multiplex aptamer selection can inexpensively provide aptamer affinity reagents to a revolutionary breadth of targets. Our team has the expertise to maximize both aptamer selection and use.